[Genetic and phenotypic analysis of heterogeneous population of a cold-adapted donor of the A/Leningrad/134/17/57 (H2N2) attenuation and of the donor-based reassortant influenza vaccine strains].
Identifieur interne : 002E24 ( Main/Exploration ); précédent : 002E23; suivant : 002E25[Genetic and phenotypic analysis of heterogeneous population of a cold-adapted donor of the A/Leningrad/134/17/57 (H2N2) attenuation and of the donor-based reassortant influenza vaccine strains].
Auteurs : I V Kiseleva ; A I Klimov ; E P Grigor'Eva ; N V Larionova ; G I Aleksandrova ; L G RudenkoSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Basse température, Fièvre (étiologie), Grippe humaine (), Grippe humaine (virologie), Génotype, Humains, Mutation, Nucléoprotéines (génétique), Phénotype, Protéines de la matrice virale (génétique), Protéines virales (génétique), Sous-type H2N2 du virus de la grippe A, Vaccination, Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (effets indésirables), Vaccins antigrippaux (génétique), Vaccins atténués (administration et posologie), Vaccins atténués (effets indésirables), Vaccins atténués (génétique), Virus de la grippe A (croissance et développement), Virus de la grippe A (génétique), Virus recombinants (croissance et développement), Virus recombinants (génétique).
- MESH :
- administration et posologie : Vaccins antigrippaux, Vaccins atténués.
- croissance et développement : Virus de la grippe A, Virus recombinants.
- effets indésirables : Vaccins antigrippaux, Vaccins atténués.
- génétique : Nucléoprotéines, Protéines de la matrice virale, Protéines virales, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A, Virus recombinants.
- virologie : Grippe humaine.
- étiologie : Fièvre.
- Adolescent, Adulte, Basse température, Grippe humaine, Génotype, Humains, Mutation, Phénotype, Sous-type H2N2 du virus de la grippe A, Vaccination.
English descriptors
- KwdEn :
- Adolescent, Adult, Cold Temperature, Fever (etiology), Genotype, Humans, Influenza A Virus, H2N2 Subtype, Influenza A virus (genetics), Influenza A virus (growth & development), Influenza Vaccines (administration & dosage), Influenza Vaccines (adverse effects), Influenza Vaccines (genetics), Influenza, Human (prevention & control), Influenza, Human (virology), Mutation, Nucleoproteins (genetics), Phenotype, Reassortant Viruses (genetics), Reassortant Viruses (growth & development), Vaccination, Vaccines, Attenuated (administration & dosage), Vaccines, Attenuated (adverse effects), Vaccines, Attenuated (genetics), Viral Matrix Proteins (genetics), Viral Proteins (genetics).
- MESH :
- chemical , administration & dosage : Influenza Vaccines, Vaccines, Attenuated.
- chemical , adverse effects : Influenza Vaccines, Vaccines, Attenuated.
- etiology : Fever.
- genetics : Influenza A virus, Influenza Vaccines, Nucleoproteins, Reassortant Viruses, Vaccines, Attenuated, Viral Matrix Proteins, Viral Proteins.
- growth & development : Influenza A virus, Reassortant Viruses.
- prevention & control : Influenza, Human.
- virology : Influenza, Human.
- Adolescent, Adult, Cold Temperature, Genotype, Humans, Influenza A Virus, H2N2 Subtype, Mutation, Phenotype, Vaccination.
Abstract
Cold-adapted (CA) temperature sensitive and attenuated virus A/Leningrad/134/17/57 (H2N2) (Len/17) has been recently used in Russia as a donor of internal genes in the preparation of reassortant vaccine strains of CA live influenza vaccine (LIV) for all age groups. The Len/17 population was found to be heterogeneous and to be made up of clones, which differ by combinations of mutations in internal genes. Around 50% of the Len/17 population had clones with all 8 coding mutations in internal genes. The others were made up of clones with mutation combinations, which were different from the original Len/17. The PCR restriction method was used to analyze 5 clones of Len/17 and 8 LIV vaccine strains. There were no Ala-86-Thr mutation in the M2 protein in 4 clones and 3 vaccine strains. The PB-1 gene of 4 clones and 3 vaccine strains had a mutation encoding Met-317-IIe more typical of a more attenuated virus A/Leningrad/134/47/57 (H2N2) (Len/47). The NP protein of a clone had a mutation Leu-341-IIe also typical of Len/47. However, neither the absence of mutation in the M2 gene nor an extra mutation in the PB1 gene affected the attenuation extent of reassortant CALIV.
PubMed: 15881391
Affiliations:
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Le document en format XML
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humaine</term><term>Génotype</term><term>Humains</term><term>Mutation</term><term>Phénotype</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cold-adapted (CA) temperature sensitive and attenuated virus A/Leningrad/134/17/57 (H2N2) (Len/17) has been recently used in Russia as a donor of internal genes in the preparation of reassortant vaccine strains of CA live influenza vaccine (LIV) for all age groups. The Len/17 population was found to be heterogeneous and to be made up of clones, which differ by combinations of mutations in internal genes. Around 50% of the Len/17 population had clones with all 8 coding mutations in internal genes. The others were made up of clones with mutation combinations, which were different from the original Len/17. The PCR restriction method was used to analyze 5 clones of Len/17 and 8 LIV vaccine strains. There were no Ala-86-Thr mutation in the M2 protein in 4 clones and 3 vaccine strains. The PB-1 gene of 4 clones and 3 vaccine strains had a mutation encoding Met-317-IIe more typical of a more attenuated virus A/Leningrad/134/47/57 (H2N2) (Len/47). The NP protein of a clone had a mutation Leu-341-IIe also typical of Len/47. However, neither the absence of mutation in the M2 gene nor an extra mutation in the PB1 gene affected the attenuation extent of reassortant CALIV.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Aleksandrova, G I" sort="Aleksandrova, G I" uniqKey="Aleksandrova G" first="G I" last="Aleksandrova">G I Aleksandrova</name><name sortKey="Grigor Eva, E P" sort="Grigor Eva, E P" uniqKey="Grigor Eva E" first="E P" last="Grigor'Eva">E P Grigor'Eva</name><name sortKey="Kiseleva, I V" sort="Kiseleva, I V" uniqKey="Kiseleva I" first="I V" last="Kiseleva">I V Kiseleva</name><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name><name sortKey="Larionova, N V" sort="Larionova, N V" uniqKey="Larionova N" first="N V" last="Larionova">N V Larionova</name><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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